Biotinylation is a biochemical technique that involves the covalent attachment of biotin (a small vitamin molecule) to proteins, nucleic acids, surfaces, or anything you can imagine. This process is facilitated by biotinylating agents, which react with specific functional groups (such as amines or sulphydryls) on the target molecules. The resulting biotinylated molecules then have a very high affinity for streptavidin. This strong binding interaction is the cornerstone of many biotechnological applications.
For researchers and biotechnologists looking to undertake their own biotinylation processes, our brief 5-step guide offers a straightforward and practical roadmap. Whether you're working on protein labelling, nucleic acid labelling, or other biotin conjugation tasks, this guide will help you achieve effective and efficient biotinylation in your lab. Follow these steps to ensure precise and reliable results in your biotinylation projects.
![Page 1 of the 5-Step Guide to Chemical Biotinylation](https://static.wixstatic.com/media/bb76a6_1d4289ee2216424bae5a8bc0e92a0100~mv2.jpg/v1/fill/w_980,h_1225,al_c,q_85,usm_0.66_1.00_0.01,enc_auto/bb76a6_1d4289ee2216424bae5a8bc0e92a0100~mv2.jpg)
![Page 2 of the 5-Step Guide to Chemical Biotinylation](https://static.wixstatic.com/media/bb76a6_365d5c90eab1480198a1398d454b9b02~mv2.jpg/v1/fill/w_980,h_1225,al_c,q_85,usm_0.66_1.00_0.01,enc_auto/bb76a6_365d5c90eab1480198a1398d454b9b02~mv2.jpg)
![Page 3 of the 5-Step Guide to Chemical Biotinylation](https://static.wixstatic.com/media/bb76a6_f0b29fa73d4449a6bd242c3295046e06~mv2.jpg/v1/fill/w_980,h_1225,al_c,q_85,usm_0.66_1.00_0.01,enc_auto/bb76a6_f0b29fa73d4449a6bd242c3295046e06~mv2.jpg)
![Page 4 of the 5-Step Guide to Chemical Biotinylation](https://static.wixstatic.com/media/bb76a6_50be2151cce847d9ac518eb9a4317879~mv2.jpg/v1/fill/w_980,h_1225,al_c,q_85,usm_0.66_1.00_0.01,enc_auto/bb76a6_50be2151cce847d9ac518eb9a4317879~mv2.jpg)
![Page 5 of the 5-Step Guide to Chemical Biotinylation](https://static.wixstatic.com/media/bb76a6_8670619959f44bdc8584c5bacff1856a~mv2.jpg/v1/fill/w_980,h_1225,al_c,q_85,usm_0.66_1.00_0.01,enc_auto/bb76a6_8670619959f44bdc8584c5bacff1856a~mv2.jpg)
![Page 6 of the 5-Step Guide to Chemical Biotinylation](https://static.wixstatic.com/media/bb76a6_340dfc11ec9b4c3aa2ac63319f3853f9~mv2.jpg/v1/fill/w_980,h_1225,al_c,q_85,usm_0.66_1.00_0.01,enc_auto/bb76a6_340dfc11ec9b4c3aa2ac63319f3853f9~mv2.jpg)
![Page 7 of the 5-Step Guide to Chemical Biotinylation](https://static.wixstatic.com/media/bb76a6_238880caf5584ae9af5092c531eb74d5~mv2.jpg/v1/fill/w_980,h_1225,al_c,q_85,usm_0.66_1.00_0.01,enc_auto/bb76a6_238880caf5584ae9af5092c531eb74d5~mv2.jpg)
At LifeSynth Solutions, we specialise in biotin conjugation services. Whether you need biotinylation for protein labelling, nucleic acid labelling, or other custom biotin conjugation, our expert team can provide high-quality, reliable results tailored to your specific needs. Our biotin labelling services are essential for a variety of research and diagnostic applications, ensuring precise and efficient biotin-protein, biotin-DNA, and biotin-antibody conjugations.
Comments